The complete amino acid sequence of yam (Dioscorea japonica) chitinase. A newly identified acidic class I chitinase.
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منابع مشابه
Complete Amino Acid Sequence of Chitinase - A from Leaves ef
peptides were put in order. Of seyen cysteine residues, six were linked by disulfide bonds (between Cys25 alld Cys74, Cys89 and Cys98, and Cys195 and Cys208); Cys176 was free. The enzyme consisted of 208 amino acid residues and had a molecular weight of 22,391. It consisted of only one polypeptide chain withellt a chitin-binding domai". The length of the chain was almost the same as that of the...
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The complete amine acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleayed with cyanogen bromide and the resulting three fragments, CBI, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CBI were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin ef reduced SLcarboxymethylated ...
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The complete amino acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleaved with cyanogen bromide and the resulting three fragments, CB1, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CB1 were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin of reduced S-carboxymethylated ...
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The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 try, ptic peptides deriyed from the redu ¢ ed and S-carboxy, methylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecu...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)88648-9